pscarlesshd dsred w (Addgene inc)
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Addgene inc
pscarlesshd dsred w
Pscarlesshd Dsred W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pscarlesshd+dsred/pScarlessHD-DsRed-w%2B+(Plasmid+%2380801)/bio_rxiv__64898__2026__03__19__713005-136-15-16
Average 94 stars, based on 8 article reviews
Pscarlesshd Dsred W, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pscarlesshd+dsred/pScarlessHD-DsRed-w%2B+(Plasmid+%2380801)/bio_rxiv__64898__2026__03__19__713005-136-15-16
Average 94 stars, based on 8 article reviews
pscarlesshd dsred w - by Bioz Stars,
2026-10
94/100 stars
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other:Article Title: A live-cell biosensor of in vivo receptor tyrosine kinase activity reveals feedback regulation of a developmental gradient Article Snippet: Fragments were assembled using InFusion assembly and transformed into Stellar competent E. coli cells (Takara Biosciences). Polymerase Chain Reaction:Article Title: CRISPR-Cas9 repair complexity in Drosophila melanogaster : NHEJ-induced deletions and HDR variability in the bantam microRNA gene Article Snippet: Other recombinant plasmids were generated using the NEBuilder® HiFi DNA Assembly Master Mix following the supplier’s instructions. .. To produce pBT- bantam -dsRED, a 6134 bp PCR fragment was amplified from pBT- bantam using primers d2729 and d2731, and assembled with a 1715 bp fragment amplified using primers d2728 and d2730 from Article Title: A missense mutation in human INSC causes peripheral neuropathy Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into Amplification:Article Title: CRISPR-Cas9 repair complexity in Drosophila melanogaster : NHEJ-induced deletions and HDR variability in the bantam microRNA gene Article Snippet: Other recombinant plasmids were generated using the NEBuilder® HiFi DNA Assembly Master Mix following the supplier’s instructions. .. To produce pBT- bantam -dsRED, a 6134 bp PCR fragment was amplified from pBT- bantam using primers d2729 and d2731, and assembled with a 1715 bp fragment amplified using primers d2728 and d2730 from Article Title: Epistasis among clustered lineage-specific adaptive amino acid substitutions in the Drosophila Trio protein Article Snippet: .. The plasmid backbone and dsRed were amplified from plasmid: Construct:Article Title: In vivo measurements of receptor tyrosine kinase activity reveal feedback regulation of a developmental gradient Article Snippet: Oligos containing the guides ( Table S1 ) were annealed and ligated using T4 ligase into BbsI digested pU6-BbsI-chiRNA vector (Addgene #45946) ( ). .. To produce the homologous repair construct for pYtag insertion, a 3xCD3ε-P2A-Clover- ZtSH2 construct was assembled from the 3xCD3ε ITAM sequence of pHR EGFR-CD3ε- FusionRed (Addgene #188626) and the ZtSH2 sequence from pHR iRFP-ZtSH2 (Addgene #188627) ( ) and then inserted into Article Title: A live-cell biosensor of in vivo receptor tyrosine kinase activity reveals feedback regulation of a developmental gradient. Article Snippet: .. Oligos containing the guides were annealed and ligated using T4 ligase into BbsI digested pU6-BbsI-chiRNA vector (Addgene #45946).92 To produce the homologous repair construct for pYtag insertion, a 3xCD3ε-P2A-Clover-ZtSH2 construct was assembled from the 3xCD3ε ITAM sequence of pHR EGFR-CD3ε-FusionRed (Addgene #188626) and the ZtSH2 sequence from pHR iRFP-ZtSH2 (Addgene #188627)37 and then inserted into Sequencing:Article Title: In vivo measurements of receptor tyrosine kinase activity reveal feedback regulation of a developmental gradient Article Snippet: Oligos containing the guides ( Table S1 ) were annealed and ligated using T4 ligase into BbsI digested pU6-BbsI-chiRNA vector (Addgene #45946) ( ). .. To produce the homologous repair construct for pYtag insertion, a 3xCD3ε-P2A-Clover- ZtSH2 construct was assembled from the 3xCD3ε ITAM sequence of pHR EGFR-CD3ε- FusionRed (Addgene #188626) and the ZtSH2 sequence from pHR iRFP-ZtSH2 (Addgene #188627) ( ) and then inserted into Article Title: A live-cell biosensor of in vivo receptor tyrosine kinase activity reveals feedback regulation of a developmental gradient. Article Snippet: .. Oligos containing the guides were annealed and ligated using T4 ligase into BbsI digested pU6-BbsI-chiRNA vector (Addgene #45946).92 To produce the homologous repair construct for pYtag insertion, a 3xCD3ε-P2A-Clover-ZtSH2 construct was assembled from the 3xCD3ε ITAM sequence of pHR EGFR-CD3ε-FusionRed (Addgene #188626) and the ZtSH2 sequence from pHR iRFP-ZtSH2 (Addgene #188627)37 and then inserted into Article Title: A missense mutation in human INSC causes peripheral neuropathy Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into Plasmid Preparation:Article Title: Epistasis among clustered lineage-specific adaptive amino acid substitutions in the Drosophila Trio protein Article Snippet: .. The plasmid backbone and dsRed were amplified from plasmid: Article Title: A missense mutation in human INSC causes peripheral neuropathy Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into Article Title: Association of RanGAP to Nuclear Pore Complex Component, RanBP2/Nup358, is Required for Pupal Development in Drosophila Article Snippet: .. Clone Assay:Article Title: A missense mutation in human INSC causes peripheral neuropathy Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into Transformation Assay:Article Title: A missense mutation in human INSC causes peripheral neuropathy Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into Selection:Article Title: A missense mutation in human INSC causes peripheral neuropathy Article Snippet: To clone the donor DNA plasmid, both upstream and downstream homology arms of dInsc were amplified from fly genomic DNA by Phusion® High-Fidelity DNA Polymerase (New England Biolab #M0530S) under optimized conditions. .. The two homology arms were then cloned into |